Last edited by Zubar
Wednesday, October 7, 2020 | History

5 edition of RNA-protein interactions found in the catalog.

RNA-protein interactions

a practical approach

  • 16 Want to read
  • 18 Currently reading

Published by Oxford University Press in Oxford, New York .
Written in English

    Subjects:
  • RNA-protein interactions -- Laboratory manuals.

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by Christopher W.J. Smith.
    SeriesPractical approach series ;, 192
    ContributionsSmith, Christopher W. J.
    Classifications
    LC ClassificationsQP624.75.P74 R573 1998
    The Physical Object
    Paginationxxv, 341 p. :
    Number of Pages341
    ID Numbers
    Open LibraryOL700896M
    ISBN 100199636508, 0199636516
    LC Control Number97049206

    RNA-protein interactions edited by Kiyoshi Nagai and Iain W Mattaj. IRL Press, , pages. $ hardcover (ISBN ); $ paperback (ISBN )Author: Joseph D. Puglisi.   The predominant methods for examining RNA-protein interactions are based on protein immunoprecipitation. These methods generally utilize antibodies to pull down the protein of interest and its associated RNA, which is reverse transcribed into cDNA, PCR amplified and sequenced [38, 54–59].Bioinformatic analysis is then used to map reads back to their transcripts of origin and identify .

    Background RNA-protein interactions (RPIs) play important roles in a wide variety of cellular processes, ranging from transcriptional and post-transcriptional regulation of gene expression to host defense against pathogens. High throughput experiments to identify RNA-protein interactions are beginning to provide valuable information about the complexity of RNA-protein interaction networks, but Cited by: The two major approaches are RNA Immunoprecipitation (RIP) and Cross-Linking Immunoprecipitation (CLIP). Both RIP and CLIP are similar to DNA-based ChIP in that they use antibodies to isolate specific nucleic acid-protein interactions. RNA:Protein Interaction Methods.

    The monograph approaches the study of protein-nucleic acid interactions in two distinctive ways. First, DNA-protein and RNA-protein interactions are presented together. Second, the first half of the book develops the principles of protein-nucleic acid recognition, whereas the second half applies these to more specialized topics.   After considering a number of different approaches to analyze RNA-protein interactions both directly in the cell (in vivo) and isolated in a test tube (in vitro), the biologists settled on an in vitro method known as RNA Bind-n-Seq (RBNS), developed four years ago by former Burge lab postdoc and co-author Nicole Lambert.


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RNA-protein interactions Download PDF EPUB FB2

RNA-Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the many experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction to various biochemical and genetic approaches, and from in-depth methods for Format: Paperback.

Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural Format: Paperback.

The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function.

In all three types of complex (protein–RNA, protein–ssDNA and protein–dsDNA) van der Waals contacts are significantly more common than hydrogen bond contacts. The van der Waals contacts representand % of the total interactions in the protein–dsDNA, protein–ssDNA and protein–RNA complexes, by: Get this from a library.

RNA-protein interactions. [Kiyoshi Nagai; Iain W Mattaj;] -- Many biological processes rely absolutely on specific interactions between RNA and protein molecules. Most obviously, the study of RNA-protein interactions is crucial to understanding the mechanisms.

"This manual is recommended to investigators with experience in molecular biological research, wishing to become introduced into the field of RNA-protein interactions. This book will also be a valuable source of reference for scientists needing general information about the theoretical basis and current methodological approaches to.

To help highlight the biological significance of RBP mediated regulation, online resources on experimentally verified protein-RNA interactions are briefly presented. Detecting RNA:protein interactions and isolating cDNA clones by Northwestern screening / Jeffrey Wilusz; 8.

three-hybrid system to detect and analyse RNA:protein interactions in vivo / Beilin Zhang, Brian Kraemer and Dhruba Sengupta; 9. RNA–Protein Interactions edited by Kiyoshi Nagai and Iain W Mattaj.

IRL Press at Oxford University Press,pages. £ paperback/£ hardback (ISBN 0 19 8/0 19 6).Cited by: 2. DNA-protein and RNA-protein interactions can have a profound effect on gene expression and the spatial and temporal localisation of mRNA within a cell.

DNA-protein interactions include those between DNA and transcription factors or other regulatory proteins. RNA-protein interactions include those between RNA and the ribosome, and other RNA-binding proteins (20,21).

It’s a book for the lab bench and the PIs office. Highly recommended." (Laurence Tiley, Microbiology Today, February, ) "The second edition of RNA-protein interaction protocols is part of the Methods in Molecular Biology series and includes detailed methods covering a wide range of approaches to characterise RNA-protein interactions.

A previous Workshop on RNA-Protein Interactions was reviewed by Wickens and Dahlberg (). RNA-Protein Recognition: Structures of RNA-Binding Proteins One of the most important recent advances in RNA-protein recognition has been the solution of several threedimensional structures of Cited by: Protein–RNA interactions are required to both transport a messenger RNA molecule into the cytoplasm of a eukaryotic cell and for formation of the translation machinery.

The rate of translation and stability of RNA are also influenced by protein–RNA interactions as well as RNA–RNA interactions.

Technology developed to better understand RNA–protein interactions is not limited to CLIP, but is also maturing on several additional fronts [11, 14].

Indeed, presented in this issue are a number of novel genome-wide experimental and analysis techniques that yield new insights into RBPology [ 9, 10, 20 – 25 ].Cited by: Protein-centric methods to study RNA-protein interactions. The predominant methods for examining RNA-protein interactions are based on protein immunoprecipitation.

These methods generally utilize antibodies to pull down the protein of interest and its associated RNA, which is reverse transcribed into cDNA, PCR amplified and sequenced [38,].Cited by: This detailed volume explores the continuing techniques of studying RNA-protein complexes and interactions as research in these areas expand.

After an introductory chapter, the book continues with ways to purify RNA-protein complexes assembled in cells or in isolated cellular extracts, methods for measuring various biochemical activities of RNA-interacting proteins or ribonucleoproteins.

RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods.

The expert contributors explore the isolation and characterization of RNA-protein complexes, the. The structures of these RNA–protein complexes are providing valuable insights into the binding modes and functional implications of these interactions.

Even the common RNA-binding domains (RBDs) and the double stranded RNA binding motifs (dsRBMs) have been shown to exhibit a plethora of binding by: Download Rna Protein Pdf search pdf books full free download online Free eBook and manual for Business, Education, Finance, Inspirational.

Get this from a library. RNA-protein interactions: a practical approach. [Christopher W J Smith;] -- In the PRACTICAL APPROACH series. RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Research into the role of RNA in cells has elucidated more interactions.

This how-to volume covers most problems of RNA-protein interactions and is organized as a laboratory manual with detailed protocols. Purpose: The purpose is to provide a laboratory manual for those wishing to conduct specific experiments.

Audience: This book is written for molecular investigators working on RNA-protein : $RNA−protein interactions influence many biological processes. Identifying the binding sites of RNA-binding proteins (RBPs) remains as one of the most fundamental and important challenges to the.

Researchers have taken an in-depth look at RNA-protein interactions, determining the sequence, structure, and context preferences of RNA-binding proteins in a high throughput manner.