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2 edition of Production of a heavy metal tolerant mutant from a sensitive strain of Bacillus megaterium KM. found in the catalog.

Production of a heavy metal tolerant mutant from a sensitive strain of Bacillus megaterium KM.

Lloyd Blackband

Production of a heavy metal tolerant mutant from a sensitive strain of Bacillus megaterium KM.

by Lloyd Blackband

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Published by University of Wolverhampton in Wolverhampton .
Written in English


Edition Notes

Dissertation (M.Sc.) - University of Wolverhampton 1995.

ID Numbers
Open LibraryOL19785610M

  Zampieri BDB, Pinto AB, Schultz L, de Oliveira MA, de Oliveira AJFC () Diversity and distribution of heavy metal-resistant bacteria in polluted sediments of the araca bay, sao sebastiao (sp), and the relationship between heavy metals and organic matter concentrations. Microb Ecol CrossRef Google ScholarCited by: 2.   The present study was conducted to determine the culturable bacterial profile from Kestopur canal (Kolkata, India) and analyze their heavy metal tolerance. In addition to daily sewage including solid and soluble wastes, a considerable load of toxic metals are released into this water body from industries, tanneries and agriculture, household as well as health by:

Heavy metals induce the sequel effects and inhibit the photosynthesis and respiration rate of plants (Karthik et al., ). Moreover, heavy metals interfere with mitochondrial functioning by impairing redox regulation and triggering the generation of excess ROS, which leads to damage in lipids biomembrane, and metabolic functions of the : Srinivas Ravi Manoj, Chinnannan Karthik, Krishna Kadirvelu, Padikasan Indra Arulselvi, Thangavel Sha.   Strain improvement-main 1. Strain Improvement By Hanif Alfavian 2. Improvement of strains selecting suitable producing strains from a natural population; manipulation of the existing genetic apparatus in a producing organism; introducing new genetic properties into the organism by recombinant DNA technology or genetic engineering.

Heavy Metals, Remediation is a main solution t protection. Bioremediation is a method, which uses indigenous micro flora or Introduction Soil is an only habitat which contains a variety of microflora and fauna it gives mechanical and nutritional support to higher plants. Heavy metals are widely used in the. Heavy-Metal Tolerance and Antibiotic Susceptibility of Red Pigmented Bacteria Isolated from Marine Environment. mussel from Sungai Merbok (Kedah) and sea cucumber from Tinggi Island of Malaysia, respectively. Isolates were routinely grown in Marine Agar (MA; Difco). Iden- tification of isolates was done by 16S rRNA sequence analysis.


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Production of a heavy metal tolerant mutant from a sensitive strain of Bacillus megaterium KM by Lloyd Blackband Download PDF EPUB FB2

The production of catalase was not affected by the presence of heavy metals, suggesting that in tolerant bacteria this enzyme is well protected and keeps its functional activity in the presence of metals.

This protection is important because in the absence of this enzyme hydrogen peroxide accumulates and may become lethal to bacteria. A heavy-metal-resistant bacterium Bacillus sp., strain EB1 was isolated from heavy-metal-contaminated soil in the southeast region of Turkey.

Based on 16S ribosomal DNA sequencing, the microorganism was closely related to Bacillus circulans. Minimal inhibitory concentrations of metals (MICs) for the bacterium were by: Three heavy metal resistant Bacillus spp were isolated from the industrial effluents from Kanpur against Cr, Pb, Hg, Hg, Cd, Zn and Co, where all the isolates exhibited high resistance to heavy Author: M Ch Sekhar Srikakolapu.

In the present investigation a strain of Bacillus megaterium BM05 was evaluated for cellulase production in submerged fermentation using wheat straw as a substrate. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerantBacillus sp.

and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr).

Drug resistance in Hg-tolerant. Bacterial strains that were highly tolerant of several heavy metals also showed resistance to a wide spectrum of antibiotics, and some strains bore plasmids of varying numbers and sizes.

A heavy metal resistant bacterium Bacillus spp. strain CPB4 was isolated from heavy metal contaminated soil in Korea and further characterised.

A heavy metal resistant bacterium Bacillus spp. strain CPB4 was isolated from heavy metal contaminated soil in Korea and further characterised. The CPB4 strain showed a high capacity for uptake of heavy metal Pb (Pb > Cd > Cu > Ni > Co > Mn > Cr > Zn) both in single and in mixed heavy metal Cited by: Primary screening of heavy metal resistant bacteria.

For the selective screening of heavy metal resistant bacteria, μg/mL of heavy metal (Lead) incorporated LB (Luria Bertani) agar plates (Peptone g/L, yeast extract, g/L, NaCl g/L, dextrose anhydrate g/L and agar g/L: pH −) were used and screened by standard pour plate method observed at 37 ° by: An Ni tolerant Bacillus subtilis produced substantial amount of IAA during stationary phase of growth in the medium and showed time-dependent increase in IAA production.

Enhanced production of IAA (55 μg mL − 1) was noticed in the presence of tryptophan ( μg mL − 1).Cited by: Abstract: Under the heavy metal polluted circumstances, microorganisms certainly have some changes in terms of species, quantity, community structure and diversity to adapt the environments.

Now, many heavy metal tolerant microbe groups have been studied. In the study, a heavy metal tolerant and psychrophilic bacterium strain from Antarctica was screened.

Introduction. Heavy metals released into the environment by technological activities pose a significant threat to the natural ecosystems and public health due to their toxicity and persistence in the food chain.Conventional processes for heavy metal removal often are neither effective nor economical, especially for diluted solutions.The utilization of biosorption for the treatment Cited by:   Bacillus megaterium strain MNSHK-1, isolated from a high-metal content site in Guanajuato, Mexico, has the intrinsic capacity to remove vanadium (V) and nickel (Ni) from a petrochemical spent catalyst, and counteract the toxic effects produced in the cell due to the presence of oxidative stress.

Since knowledge of the molecular components involved in the microbial resistance to Cited by: 4. Keywords: Heavy metals, Metal resistance genes, bacteria. _____ INTRODUCTION Microbes may play a large role in the biogeochemical cycling of toxic heavy metals also in cleaning up metal-contaminated.

Heavy metals are often defined as a group of metals whose atomic density is greater than 5g cm-3 [1]. A heavy metal resistant bacterium Bacillus spp. strain CPB4 was isolated from heavy metal contaminated soil in Korea and further characterised.

The CPB4 strain showed a high capacity for uptake of heavy metal Pb (Pb > Cd > Cu > Ni > Co > Mn > Cr > Zn) both in single and in mixed heavy metal Cited by: metal tolerant bacteria from industrial effluents and agricultural areas in and around Kochi, Kerala and determination of Minimum Inhibitory Concentration (MIC) and antibiotic resistance of the heavy metal resistant bacterial Size: KB.

This study aimed to evaluate three heavy metal tolerant bacterial strains (HMTB) namely Alcaligenes faecalis MG, Bacillus cereus MG and Alcaligenes faecalis MG as antioxidant agents and plant growth promotors under laboratory conditions.

Their abilities to inhibit 2,2-DiPhenylPicrylhydrazyl hydrate (DPPH) as non-enzymatic antioxidant activity and produce plant growth Cited by: 1. different sites in Indian Ocean Equator Region through primary enriching method.

Amongst, six bacteria were screened as heavy metal resistant bacteria and remaining 14 strains were sensitive. This result revealed that the bacteria tolerance to heavy metal was recorded in low number in this region compared to sensitive one.

The. In general, the Mixed Culture Consortium (MCC) of the five heavy metal-tolerant bacteria showed the highest efficacy for heavy metal reduction in the effluents. The efficacy of removal of the four heavy metals in decreasing order was MCC>P.

aeruginosa>P. vulgaris>>K. pneumoniae>S. aureus. Bacillus megaterium strain MNSHK-1, isolated from a high-metal content site in Guanajuato, Mexico, has the intrinsic capacity to remove vanadium (V) and nickel (Ni) from a petrochemical spent.

Farming and aquaculture as sources of heavy metals. The anthropogenic contamination of the environment with heavy metals is a serious problem. Aquaculture (Burridge et al., ) and agricultural practices (Han et al., ; Nicholson et al., ) contribute to this world wide pollution due to diverse applications of metals in feed additives, organic and inorganic fertilizers, pesticides, and Cited by: Microbial immobilization is considered as a novel and environmentally friendly technology that uses microbes to reduce heavy metals accumulation in plants.

To explore microbial resources which are useful in these applications, three water spinach rhizosphere soils polluted by different levels of heavy metals (heavy pollution (CQ), medium pollution (JZ), and relative clean (NF)) were collected.

SUMMARY Endospores of Bacillus spp., especially Bacillus subtilis, have served as experimental models for exploring the molecular mechanisms underlying the incredible longevity of spores and their resistance to environmental insults. In this review we summarize the molecular laboratory model of spore resistance mechanisms and attempt to use the model as a basis for Cited by: